General Cell Based Assays
Enzo Life Sciences Matrix Metalloproteinase-3 (MMP-3) colorimetric drug discovery kit (1x96wells)
A QUANTIZYME Assay System. The MMP-3 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-3 (MMP-3, stromelysin-1) using a thiopeptide as a chromogenic substrate. The assays are performed in a convenient 96 well microplate format. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH), and a detailed instruction booklet. Alternative name: Stromelysin 1, Transin 1. Applications: Colorimetric detection, HTS Activity assay.
Enzo Life Sciences Matrix Metalloproteinase-3 (MMP-3) fluorometric drug discovery kit (1x96wells)
A QUANTIZYME Assay System. The MMP-3 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-3 (MMP-3, stromelysin-1) using a quenched fluorogenic peptide: OMNIMMP fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-3, a potential therapeutic target. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet. Alternative name: Stromelysin 1, Transin 1. Applications: Fluorescent detection, HTS Activity assay.
Enzo Life Sciences Matrix Metalloproteinase-12 (MMP-12) fluorometric drug discovery kit (1x96wells)
A QUANTIZYME Assay System. The MMP-12 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-12 (MMP-12, metalloelastase, macrophage elastase) using a quenched fluorogenic peptide: OMNIMMP fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-12, a potential therapeutic target. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet. Alternative name: Metalloelastase, Macrophage elastase. Applications: Fluorescent detection, HTS Activity assay.
Enzo Life Sciences Matrix metalloproteinase-12 (MMP-12) fluorometric drug discovery kit, GREEN (1x96wells)
The MMP-12 Fluorometric (also known as fluorimetric) Drug Discovery Kit, GREEN is a complete assay system designed to screen MMP-12 inhibitors using a quenched fluorogenic OMNIMMP Green substrate: 5-FAM-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(TQ2W)-NH2 [5-FAM = 5-carboxyfluorescein; Nva=norvaline; TQ2W=quencher]. FAM fluorescence is thoroughly quenched by the TQ2W group until cleavage by MMPs separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-12, a potential therapeutic target. The compound NNGH is also included as a prototypic control inhibitor. Alternative name: Metalloelastase, Macrophage elastase. Applications: Fluorescent detection, HTS Activity assay.
Enzo Life Sciences Matrix metalloproteinase-3 (MMP-3) fluorometric drug discovery kit, GREEN (1x96wells)
The MMP-3 Fluorometric (also known as fluorimetric)Drug Discovery Kit, GREEN is a complete assay system designed to screen MMP-3 inhibitors using a quenched fluorogenic MMP-3 substrate: 5-FAM-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(TQ2W)-NH2 [5-FAM=5-carboxyfluorescein; Nva=norvaline; TQ2W=quencher]. FAM fluorescence is thoroughly quenched by the TQ2W group until cleavage by MMPs separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-3, a potential therapeutic target. The compound NNGH is also included as a prototypic control inhibitor. Alternative name: Stromelysin 1, Transin 1. Applications: Fluorescent detection, HTS Activity assay.
Enzo Life Sciences CV-Cathepsin L detection kit (1 Kit)
Elevated cathepsin enzyme activity often indicates the presence of a pathological condition including cancer, Alzheimer's disease, autoimmune disease, and osteoporosis. Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy. Once cells are grown, staining is completed in just a few minutes. Hoechst 33342 stain and Acridine Orange are also included to allow visualization of nuclei and lysosomes. Applications: Fluorescence microscopy, Fluorescent detection .
Eurofins Discoverx cAMP Hunter eXpress PTGER3 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0159E2CP2M)
The cAMP Hunter PTGER3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2, cloprostenol) based activation of PTGER3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTGER3, a naturally Gi/Go coupled GPCR. Upon activation of PTGER3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences CHEMILUM DE LYS HDAC/SIRT Chemiluminescent drug discovery kit (1x96wells)
The HDAC/SIRT Chemiluminescent Drug Discovery Kit is a complete assay system designed to measure histone deacetylase and sirtuin activity in cell or nuclear extracts, immunoprecipitates or purified enzymes. The HDAC Chemiluminescent Activity Assay is based on the unique CHEMILUM DE LYS Substrate and Developer combination. The CHEMILUM DE LYS system is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has three steps. First, the CHEMILUM DE LYS Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity . Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the CHEMILUM DE LYS Developer followed by Enhancer produces light. The reaction is luciferase-free Alternative name: Histone deacetylase fluorescent assay kit. Applications: Chemiluminescence, HTS Activity assay.
Enzo Life Sciences Cathepsin K drug discovery kit (1x96wells)
The Cathepsin K Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin K. Using a convenient microplate format, cathepsin K activity is monitored fluorimetrically using the substrate provided in the kit. Applications: Fluorescent detection, HTS Activity assay.
Med Vet International Small Animal Fecal Assay Starter Kit, J1101SD
1 gallon Sheather's Sugar Solution Dosing syringe for fecal solution Microscope slides and cover slips Pet mitts for stool collection Wash bottle Fecal assay strainer Test tube rack Handheld tally counter Centrifuge tubes Surgical scissors Tongs
Meso Scale Discovery MAP Kinase WCL20ate
The MAP Kinase Whole Cell Lysate Kit from Meso Scale Discovery provides reagents to detect total and phosphorylated ERK1/2 JNK1/2 and p38 MAP kinases in cell lysates The kit contains a 96-well plate coated with capture antibodies detection antibodies and buffers needed to perform the assay The electrochemiluminescent detection allows for high sensitivity and a wide dynamic range This kit allows for the quantitative analysis of MAP kinase activation in cells With a single 96-well plate up to 40 samples can be analyzed in triplicate
Enzo Life Sciences Cell Counting Kit-F (500 tests)
Fluorometric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages: No radioisotopes, more sensitive than the colorimetric assay, short staining reaction time, no solubilization step, correlates with the [3H]-thymidine incorporation assay. Alternative name: CCKi-F. Applications: Fluorescence microscopy .
Enzo Life Sciences Cell Counting Kit-F (500 tests)
Fluorometric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages: No radioisotopes, more sensitive than the colorimetric assay, short staining reaction time, no solubilization step, correlates with the [3H]-thymidine incorporation assay. Alternative name: CCKi-F. Applications: Fluorescence microscopy .